Zebrafish Larvae Tracking & Behavioral Analysis

Automated deep-learning software for high-throughput tracking and behavioral analysis of zebrafish larvae in toxicology and drug-discovery studies.

Behavioral Analysis & ESLT Methodology

The Electrical Stimulation Locomotor Test (ESLT) triggers escape swims independently of sensory functions, enabling reproducible measurement of neuromuscular circuits and toxicant-specific behavioral defects.

01

High-Throughput ESLT Assay

Assess pre-feeding zebrafish larvae after standardized electrical stimulation, with consistent acquisition for toxicological and pharmacological screening.

ESLT4–7 dpf
02

FishNet Tracking

Detect multiple larvae, locate the head, reconstruct the body midline, and follow complex interactions including crossing fish.

Deep LearningMulti-fish
03

Behavioral Modules

Bring together neuromuscular, postural control, and epileptiform seizure-related measurements into an integrated behavioral profile.

KinematicsPosture
04

EpilepsyNet Classifier

Identify epileptiform seizure behavior and balance loss from FishNet-derived neuromuscular parameters to support antidote and drug screening.

SeizuresDrug Screening
Measurement Modules

Three independent behavioral pathways

Separate neuromuscular function, postural control, and epileptiform seizure characterization to reveal distinct modes of action.

Neuromuscular FunctionSpeed · latency · duration
01
Postural ControlBalance · rolling
02
Seizure CharacterizationEpileptiform activity
03
Compound ProfilingToxicants · therapeutics
04

See DanioTracker in action

Preview the acquisition, tracking, and seizure-analysis workflow through representative zebrafish videos.

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01

High-throughput acquisition

Standardized ESLT acquisition captures group swimming responses for robust, repeatable screening.

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02

Accurate multi-fish tracking

FishNet maintains individual tracks and body geometry even when larvae cross or interact.

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03

Epileptiform behavior

EpilepsyNet identifies seizure-like events and produces quantitative profiles for therapeutic screening.

Desktop Application
DanioTracker Logo

Download DanioTracker

The desktop release will package the FishNet and EpilepsyNet workflow for local, high-throughput behavioral analysis.

Downloads are temporarily unavailable. DanioTracker download will open after publication.

DanioTracker
Release forthcoming
System requirements
  • Windows 10/11 x64
  • >8 GB RAM
  • NVIDIA GPU recommended
Video requirements
  • .mp4, .avi, or .webm
  • 720p or higher
  • 60–1000 fps preferred

Credits & Institutional Partners